Light is necessary for life, but long exposure to artificial light is becoming a major health concern.
Humans are exposed to increased amounts of light in the blue spectrum produced by light-emitting diodes (LEDs) found in screens and smartphones which can interfere with biological processes.
The LED technologies are relatively new and the long-term effects of exposure to blue light across the lifespan are not yet understood.
DermoMeca® proposes to study physical behavior of fibroblasts, cells responsible for collagen and elastin synthesis, and dermis sustaining capacity.
Biological models :
- in vitro models : cells grown on collagen lattice
- ex vitro models : reconstructed skin models, skin explants, etc.
- Cell morphometry (area, perimeter, circularity, aspect ratio, etc.)
- Cell mechanical properties (stiffness, elastic modulus, traction force)
- Morphometric parameters of collagen network (fiber density, fiber thickness, fiber orientation)
- Morphometric parameters of collagen fibers (thickness, period measurement, etc.)
- Network mechanical properties (tension, strain stiffening)
Figure 1 : A. AFM topographical image of collagen I polymerized during 18h.
B. AFM topographical image of collagen I polymerized during 18h under blue light (800J/cm²).
C. Analysis of collagen I fibers tickness on both conditions.
D. Elastic modulus of collagen I lattice in both conditions.