Stem integrity in Arabidopsis thaliana requires a load-bearing

Mariko Asaoka, Mao Ooe, Shizuka Gunji, Pascale Milani, Gaël Runel, Gorou Horiguchi,Olivier Hamant, Shinichiro Sawa, Hirokazu Tsukaya and Ali Ferjani.


Because plant cells are glued to each other via their cell walls, failure to coordinate growth among adjacent cells can create cracks in tissues. Here, we find that the unbalanced growth of inner and outer tissues in the clavata3 de-etiolated3 (clv3 det3) mutant of Arabidopsis thaliana stretched epidermal cells, ultimately generating cracks in stems. Stem growth slowed before cracks appeared along clv3 det3 stems, whereas inner pith cells became drastically distorted and accelerated their growth, yielding to stress, after the appearance of cracks. This is consistent with a key role of the epidermis in restricting growth. Mechanical property measurements recorded using an atomic force microscope revealed that epidermal cellwall stiffness decreased in det3 and clv3 det3 epidermises. Thus, we hypothesized that stem integrity depends on the epidermal resistance to mechanical stress. To formally test this hypothesis,we used the DET3 gene as part of a tissue-specific strategy to complement cell expansion defects. Epidermis-driven DET3 expression restored growth and restored the frequency of stemcracking to 20% of the clv3 det3 mutant, demonstrating the DET3-dependent load-bearing role of the epidermis.

Vous souhaitez en savoir plus ?

Télécharger notre publication scientifique en intégralité !

Stiffness measurement is a biomarker of skin aging in vivo

Runel G, Cario M, Lopez-Ramirez N, Malbouyres M, Ruggiero F, Bernard L, Puisieux A, Caramel J, Chlasta J, Masse I.


Biomechanical Properties of Cancer Cells

Runel G, Lopez-Ramirez N, Chlasta J, Masse I.


Consult the abstract

Gradient in cytoplasmic pressure in germline cells controls overlying epithelial cell morphogenesis

Lamiré L-A, Milani P, Runel G, Kiss A, Arias L, Vergier B, de Bossoreille S, Das P, Cluet D, Boudaoud A, Grammont M. November 30, 2020.


Consult the abstract

Gene profile of zebrafish fin regeneration offers clues to kinetics, organization and biomechanics of basement membrane.

Nauroy P, Guiraud A, Chlasta J, Malbouyres M, Gillet B, Hughes S, Lambert E, Ruggiero F. Matrix Biology


Changes in nano-mechanical properties of human epidermal cornified cells depending on their proximity to the skin surface

Milani P, Chlasta J, Abdayem R, Kezic S, Haftek M. J Mol Recognit. 22 mai 2018;e2722.


Consult the abstract

Variations in basement membrane mechanics are linked to epithelial morphogenesis

Chlasta J, Milani P, Runel G, Duteyrat JL, Arias L, Lamiré LA, Boudaoud A, Grammont M. Development 2017 : doi: 10.1242/dev.152652


Consult the abstract

Stromal protein βig-h3 reprogrammes tumour microenvironment in pancreatic cancer

Goehrig D, Nigri J, Samain R, Wu Z, Cappello P, Gabiane G, Zhang X, Zhao Y, Kim IS, Chanal M, Curto R, Hervieu V, de la Fouchardière C, Novelli F, Milani P, Tomasini R, Bousquet C, Bertolino P, Hennino A.


Consult the abstract

Mechanical Shielding in Plant Nuclei

Goswami R, Asnacios A, Milani P, Graindorge S, Houlné G, Mutterer J, Hamant O, Chabouté M-E.


Consult the abstract

Changes in nano-mechanical properties of human epidermal cornified cells in children with atopic dermatitis

Haftek M, McAleer MA, Jakasa I, Irwin McLean WH, Kezic S, Irvine AD.


Consult the abstract

KATANIN-dependent mechanical properties of the stigmatic cell wall mediate the pollen tube path in Arabidospis

Riglet L, Rozier F, Kodera C, Bovio S, Sechet J, Fobis-Loisy I, Gaude T.


Consult the abstract