Images of the month

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Desquamation

May 2022

Skin explant topography performed by high-resolution AFM imaging. Some corneocytes involved in the desquamation process are visible (arrows). 
Scale bar: 20µm.

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culture of fibroblasts in collagen discs, allowing the culture of fibroblasts in a three dimensional system, alternative to the classical two dimensional culture system

Fibroblasts

 

September 2021

Culture of fibroblasts in collagen discs, allowing the culture of fibroblasts in a three dimensional system, alternative to the classical two dimensional culture system.

Nuclear (green) and actin cytoskeleton (red) labeling.

 

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3D force map of dermal-epidermal junction (DEJ) evolution over time with Atomic Force Microscopy (AFM)

Dermal-epidermal junction

June 2021

3D force map of dermal-epidermal junction (DEJ) evolution over time with Atomic Force Microscopy (AFM).

Each pixel corresponds to a stiffness value:
🟢 indicates a low stiffness
🔴 indicates a high stiffness
🟠 indicates an intermediate value
A new solution to assess skin ageing focusing on DEJ.

 

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AFM topographic image of skin from abdominoplasty, highlighting the main skin compartments

Skin compartments

February 2021

AFM topographic image of skin from abdominoplasty, highlighting the main skin compartments:
👉 At the top of the picture, epidermis with its 3 layers (granulosa, spinous and basal) and at the very top, stratum corneum.
👉 Dermis, at the bottom of the picture, with above epidermis-dermis junction-containing area.

 

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Fibroblasts

November 2020

High-resolution image of a fibroblast using confocal microscopy (for phalloidin labeling of actin) and second harmonic microscopy (for collagen).

Carried out on an uncoated petri dish, with a collagen neo-synthesised by fibroblast.

 

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Skin aging process

July 2020

Microcirculation study through skin capillaries high resolution imaging by light sheet fluorescence microscopy in order to better understand skin aging process.

 

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Wound healing

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