To characterize a transmembrane protein by High Resolution Imaging and localize its binding sites, BioMeca® provides 2 solutions :
- Force-Distance curve-based Atomic Force Microscopy (FD-based AFM) technology for High Resolution Imaging,
- AFM tips functionalization with a tris-Ni+-NTA groups, to detect the specific protein binding sites, at the nanometer resolution.
These technologies allow BioMeca® to quantify the blinding strength of tris-Ni+-NTA groups to histidine residues and validate the number of TransMembrane Domains (TMD) in this protein receptor of
A : Mapping adhesion forces on membrane proteins using FD-based AFM. When recording an AFM topograph an approach (blue) and retraction (yellow) cycle between AFM tip and biological sample is performed for every pixel of the image. In each cycle, the cantilever deflection and the distance travelled by the AFM tip is monitored and transformed into an approach and retraction Force Distance (FD) curve
B : Functionalization of AFM tip (tris-Ni2+-NTA)
High resolution AFM image of two proteoliposomes immobilized on a mica surface. 1: proteoliposome 1, 2 : proteoliposome 2.